Genome Engineering
Prof. Dr. Wolfgang Wurst
Gruppenleiter (kommissarisch)
Die Core Facility „Transgene Einheit“ und „Verhaltensanalyse von Mausmodellen“ sind dem Lehrstuhl von Prof. Wurst (HMGU; TU) assoziiert
Ingolstädter Landstr. 1
85764 Oberschleißheim-Neuherberg

wolfgang.wurst@dzne.de
 +49 89 3187-4111

Publikationen

Die vollständige Publikationsliste findet man hier.

Meehan TF, Conte N, West DB, Jacobsen JO, Mason J, Warren J, Chen CK, Tudose I, Relac M, Matthews P, Karp N, Santos L, Fiegel T, Ring N, Westerberg H, Greenaway S, Sneddon D, Morgan H, Codner GF, Stewart ME, Brown J, Horner N; International Mouse Phenotyping Consortium, Haendel M, Washington N, Mungall CJ, Reynolds CL, Gallegos J, Gailus-Durner V, Sorg T, Pavlovic G, Bower LR, Moore M, Morse I, Gao X, Tocchini-Valentini GP, Obata Y, Cho SY, Seong JK, Seavitt J, Beaudet AL, Dickinson ME, Herault Y, Wurst W, de Angelis MH, Lloyd KCK, Flenniken AM, Nutter LMJ, Newbigging S, McKerlie C, Justice MJ, Murray SA, Svenson KL, Braun RE, White JK, Bradley A, Flicek P, Wells S, Skarnes WC, Adams DJ, Parkinson H, Mallon AM, Brown SDM, Smedley D. Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium. Nat Genet. 2017 Aug 01; 49:1231-1238. doi: 10.1038/ng.3901
Gernot Kleinberger, Matthias Brendel, Eva Mracsko, Benedikt Wefers, Linda Groeneweg, Xianyuan Xiang, Carola Focke, Maximilian Deußing, Marc Suárez-Calvet, Fargol Mazaheri, Samira Parhizkar, Nadine Pettkus, Wolfgang Wurst, Regina Feederle, Peter Bartenstein, Thomas Mueggler, Thomas Arzberger, Irene Knuesel, Axel Rominger, Christian Haass. The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism. EMBO Journal. 2017 Jul 02; 36:1837-1853. doi: 10.15252/embj.201796516
Fargol Mazaheri, Nicolas Snaidero, Gernot Kleinberger, Charlotte Madore, Anna Daria, Georg Werner, Susanne Krasemann, Anja Capell, Dietrich Trümbach, Wolfgang Wurst, Bettina Brunner, Sebastian Bultmann, Sabina Tahirovic, Martin Kerschensteiner, Thomas Misgeld, Oleg Butovsky, Christian Haass. TREM2 deficiency impairs chemotaxis and microglial responses to neuronal injury. EMBO Reports. 2017 Jun 30; 18:1186-1198. doi: 10.15252/embr.201743922
Eric Danner, Sanum Bashir, Saniye Yumlu, Wolfgang Wurst, Benedikt Wefers, Ralf Kühn. Control of gene editing by manipulation of DNA repair mechanisms. Mammalian Genome. 2017 Apr 02; 28:262-274. doi: 10.1007/s00335-017-9688-5
Benedikt Wefers, Sanum Bashir, Jana Rossius, Wolfgang Wurst, Ralf Kühn. Gene editing in mouse zygotes using the CRISPR/Cas9 system. Methods. 2017 May 14; 121-122:55-67. doi: 10.1016/j.ymeth.2017.02.008
Benedikt Wefers, Christina Brandl, Oskar Ortiz, Wolfgang Wurst, Ralf Kühn. Genome Editing in Mice Using TALE Nucleases. Methods in molecular biology (Clifton, N.J.). 2015 Dec 31; 1338:229-243. doi: 10.1007/978-1-4939-2932-0_17
Joel A. Schick, Claudia Seisenberger, Joachim Beig, Antje Bürger, Vivek Iyer, Viola Maier, Sajith Perera, Barry Rosen, William C. Skarnes, Wolfgang Wurst. CRISPR-Cas9 enables conditional mutagenesis of challenging loci. Scientific Reports. 2016 Aug 31; 6 doi: 10.1038/srep32326
Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kühn R. Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 May 01; 33:543-8. doi: 10.1038/nbt.3198
Rosen, Schick, Wurst. Beyond knockouts: the International Knockout Mouse Consortium delivers modular and evolving tools for investigating mammalian genes. Mammalian Genome. 2015 Sep 30; 26:456-466. doi: 10.1007/s00335-015-9598-3
Christina Brandl, Oskar Ortiz, Bernhard Röttig, Benedikt Wefers, Wolfgang Wurst, Ralf Kühn. Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one-cell mouse embryos. FEBS Open Bio. 2014 Dec 31; 5:26-35. doi: 10.1016/j.fob.2014.11.009
Benedikt Wefers, Sudeepta K. Panda, Oskar Ortiz, Christina Brandl, Svenja Hensler, Jens Hansen, Wolfgang Wurst, Ralf Kühn. Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA. Nature Protocols. 2012 Dec 31; 8:2355-2379. doi: 10.1038/nprot2013142
Panda SK, Wefers B, Ortiz O, Floss T, Schmid B, Haass C, Wurst W, Kühn R. Highly efficient targeted mutagenesis in mice using TALENs. Genetics. 2013 Nov 01; 195:703-13. doi: 10.1534/genetics.113.156570
Wefers B, Meyer M, Ortiz O, Hrabé de Angelis M, Hansen J, Wurst W, Kühn R. Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides. Proc Natl Acad Sci U S A. 2013 Mar 05; 110:3782-7. doi: 10.1073/pnas.1218721110
Allan Bradley, Konstantinos Anastassiadis, Abdelkader Ayadi, James F. Battey, Cindy Bell, Marie-Christine Birling, Joanna Bottomley, Steve D. Brown, Antje Bürger, Carol J. Bult, Wendy Bushell, Francis S. Collins, Christian Desaintes, Brendan Doe, Economides Aris, Janan T. Eppig, Richard H. Finnell, Colin Fletcher, Martin Fray, David Frendewey, Roland H. Friedel, Frank G. Grosveld, Jens Hansen, Yann Hérault, Geoffrey Hicks, Andreas Hörlein, Richard Houghton, Martin Hrabé De Angelis, Danny Huylebroeck, Vivek Iyer, Pieter J. De Jong, James A. Kadin, Cornelia Kaloff, Karen Kennedy, Manousos Koutsourakis, Kent Lloyd, Susan Marschall, Jeremy Mason, Colin McKerlie, Michael P. McLeod, Harald Von Melchner, Mark Moore, Alejandro O. Mujica, Andras Nagy, Mikhail Nefedov, Lauryl M. Nutter, Guillaume Pavlovic, Jane L. Peterson, Jonathan Pollock, Ramiro Ramirez-Solis, Derrick E. Rancourt, Marcello Raspa, Jacques E. Remacle, Martin Ringwald, Barry Rosen, Nadia Rosenthal, Janet Rossant, Patricia Ruiz Noppinger, Ed Ryder, Joel Zupicich Schick, Frank Schnütgen, Paul Schofield, Claudia Seisenberger, Mohammed Selloum, Elizabeth M. Simpson, William C. Skarnes, Damian Smedley, William L. Stanford, Francis Stewart, Kevin Stone, Kate Swan, Hamsa Tadepally, Lydia Teboul, Glauco P. Tocchini-Valentini, David Valenzuela, Anthony P. West, Ken-Ichi Yamamura, Yuko Yoshinaga, Wolfgang Wurst. The mammalian gene function resource: The International Knockout Mouse Consortium. Mammalian Genome. 2012 Sep 30; 23:580-586. doi: 10.1007/s00335-012-9422-2
International Mouse Knockout Consortium, Collins FS, Rossant J, Wurst W. A mouse for all reasons. Cell. 2007 Jan 12; 128:9-13. doi: 10.1016/j.cell.2006.12.018

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