Sabina Tahirovic
Ex vivo models
Dr. Sabina Tahirovic
Group Leader
Feodor-Lynen-Str. 17
81377  München

sabina.tahirovic@dzne.de
 +49 89 4400-46438

Areas of investigation/research focus

Our research focuses on microglial dysfunction and alterations in neuronal and glial signaling contributing to the pathogenesis of neurodegenerative disorders like Alzheimer´s disease (AD), Niemann-Pick type C (NPC) and Frontotemporal lobar degeneration (FTLD). Better understanding of cell-specific pathological changes and cross-talk between different brain cells will help us to reveal novel therapeutic targets for medical intervention.

Microglial activation is one of the key pathological hallmarks of neurodegeneration. It is proposed that in AD microglia are inefficient in removing ß-amyloid deposits. To study microglial contribution to amyloid plaque burden, we designed a novel ex vivo model (brains in a dish) where we culture together organotypic brain slices from aged, amyloid-bearing and young wild-type mice. The co-culture paradigm or only the exposure of old AD microglia to soluble factors produced by young microglia was sufficient to “rejuvenate” their phagocytic capacity and trigger amyloid plaque clearance. Our goal is to identify compounds aimed to instruct AD microglia to clear amyloid and understand how microglial immunomodulation can be used to mitigate neuropathology. A future challenge will be to therapeutically enhance beneficial responses of microglia -such as phagocytosis- to ameliorate AD.

 more Infos

Beyond AD, microglial contribution becomes appreciated in other neurodegenerative conditions such as FTLD and NPC. Those diseases are of particular interest for our research due to common mechanisms shared between lysosomal storage disorders and neurodegeneration. NPC is a lysosomal storage disorder that is due to many shared pathological features also called juvenile AD as it is mainly affecting children and young adults. Our research goal is to characterize microglial dysfunction and dissect common and disease-specific molecular alterations in AD, FTLD and NPC.

We combine our expertise in isolation and characterization of primary cells (neurons, astrocytes, microglia and organotypic brain slices) with in vivo approaches (transgenic mouse models of neurodegeneration) to elucidate complex pathological changes of different brain cells. Using cell biological, biochemical, immunohistochemical and proteomic analysis we asses the contribution of cell-type-specific alterations to disease onset and progression with the final aim to identify potential drug targets for treatment of those devastating neurodegenerative disorders.

Key Publications

Monasor LS, Müller SA, Colombo A, König J, Roth S, Liesz A, Berghofer A, Saito T, Saido TC, Herms J, Willem M, Haass C, Lichtenthaler SF, Tahirovic S. Fibrillar Aβ triggers microglial proteome alterations and dysfunction in Alzheimer mouse models. Elife. 2020 Jan 01; 9
Samira Parhizkar, Thomas Arzberger, Matthias Brendel, Gernot Kleinberger, Maximilian Deussing, Carola Focke, Brigitte Nuscher, Monica Xiong, Alireza Ghasemigharagoz, Natalie Katzmarski, Susanne Krasemann, Stefan F. Lichtenthaler, Stephan A. Müller, Alessio Colombo, Laura Sebastian Monasor, Sabina Tahirovic, Jochen Herms, Michael Willem, Nadine Pettkus, Oleg Butovsky, Peter Bartenstein, Dieter Edbauer, Axel Rominger, Ali Ertürk, Stefan A. Grathwohl, Jonas J. Neher, David M. Holtzman, Melanie Meyer-Luehmann, Christian Haass. Loss of TREM2 function increases amyloid seeding but reduces plaque-associated ApoE. Nature Neuroscience. 2019 Jan 31; 22:191-204. doi: 10.1038/s41593-018-0296-9
Claes C, Van Den Daele J, Boon R, Schouteden S, Colombo A, Monasor LS, Fiers M, Ordovas L, Nami F, Bohrmann B, Tahirovic S, De Strooper B, Verfaillie CM. Human stem cell-derived monocytes and microglia-like cells reveal impaired amyloid plaque clearance upon heterozygous or homozygous loss of TREM2. Alzheimers Dement. 2019 Jan 01; 15:453-464.
Julia K. Götzl, Alessio-Vittorio Colombo, Katrin Fellerer, Anika Reifschneider, Georg Werner, Sabina Tahirovic, Christian Haass, Anja Capell. Early lysosomal maturation deficits in microglia triggers enhanced lysosomal activity in other brain cells of progranulin knockout mice. Molecular Neurodegeneration. 2018 Sep 03; 13 doi: 10.1186/s13024-018-0281-5
Anna Daria, Alessio Colombo, Gemma Llovera, Heike Hampel, Michael Willem, Arthur Liesz, Christian Haass, Sabina Tahirovic. Young microglia restore amyloid plaque clearance of aged microglia. EMBO Journal. 2017 Feb 28; 36:583-603. doi: 10.15252/embj.201694591

Info-Hotline

Thursdays 1:30-4:30 pm

Patients +49 800-7799001

(free of charge)

Professionals +49 180-779900

(9 Cent/Min. German landline, mobile and out of Germany possibly more expensive)

Welcome to our website, here you can inform yourself basically cookie-free.

We would be pleased if you would allow a cookie to be set for analysis purposes in order to optimise our provided information. All data are pseudonymous and are only used by the DZNE. We deliberately avoid third-party cookies. You can deselect this setting at any time here.

Your browser allows the setting of cookies: