Platform foR SinglE Cell GenomIcS and Epigenomics (PRECISE)
Single cell genomics is entirely changing the way how we are characterizing cellular responses in homeostasis and many common diseases. In fact, the cellular landscapes will be re-written for many complex organs and diseases.
Platform for Single Cell Genomics and Epigenomics
The "Platform for Single Cell Genomics and Epigenomics" is a joint Venture between the University of Bonn and the German Center for Neurodegenerative Diseases (DZNE). Both institutions seek to conjointly increase their expert knowledge in the areas of genomics and bioinformatics. Particular focus lies on the development and application of tools and technologies for single-cell genomics and on providing these technologies for internal and external collaborators.
The laboratory space of the platform is situated in the new DZNE building B, the bioinformatics unit is located at the LIMES Institute. Both units are closely collaborating.
Some of the technologies offered at PRECISE are listed below:
Single-cell transcriptomics is a rapid moving field, where new library preparation and computational methods are introduced every few weeks. It is therefore vital to keep up with the pace of technological development. We are investing important amounts of time, human and technical resources in testing and implementing recently released new single-cell technologies as well as developing better, faster and inexpensive methods to be able to offer the most cutting-edge technologies available and tackle ever more complex biological questions.
Some of the new technologies in the pipeline are listed below:
Equipment of the platform
Liquid handling and QC systems
- BioNex Nanodrop II
- Dispendix I.Dot
- SPT Labtech Mosquito x1
- Tecan Freedom EVO
- Roche Lightcycler 480
- Agilent Tapestation 4200
- Agilent Fragment Analyzer
- Thermo Fisher Qubit 3, Flex
NGS library generation
- BD Rhapsody HT Xpress, Scanner and Station
- 10X Genomics Chromium and Chromium iX
- Cytena C.Wash
- Covaris LE220
- Diagenode IP-Star
- Pippin Prep Instrument
Sequencing instruments
- Illumina MiSeq
- Illumina NextSeq 2000
- Illumina NovaSeq 6000
- Illumina NovaSeq X Plus
- PacBio Sequel II
Spatial transcriptomics
- Nanostring GeoMx Digital Spatial Profiler
- 10X Xenium Analyzer
Proteomics
- Alamar Argo HT