Two-Photon Microscopes

Two-photon microscopy uses two photons in the infrared spectral range instead of one photon in the visible spectral range to excite fluorophores. Due to less scattering of infrared light this microscopy technique can image much deeper into the tissue than conventional “single”-photon techniques. In addition, two-photon microscopes excite fluorophores only in the plane of focus and thus provide z-resolution comparable to confocal microscopy. Two-photon-microscopes are therefore ideally suited for all kinds of photo-manipulation experiments.

 

Zeiss LSM710NLO

Zeiss LSM710NLO

This microscope is both a two-photon- and a confocal microscope. For details please see section Confocal Laser Scanning Microscopes.

TRIM Scopell with OPO

TRIM ScopeII with OPO

Our LaVision Trim ScopeII with OPO is an upright two-photon setup with an infrared laser, four non-descanned detectors (NDDs), and a high sensitive EM-CCD camera. NDD’s and camera are in the reflection mode. Two NDDs are ultra sensitive gallium arsenide phosphide (GaAsP) detectors. The laser wavelength can be tuned into the far infrared region with an optical  parametric oscillator (OPO). Furthermore, the system is equipped with LaVision’s 64 beam technology for high speed two-photon microscopy. This technology uses 64 laser beams instead of one to scan the sample and an EM-CCD camera to detect the fluorescence. The setup is ideally suited for in vivo imaging of narcotized animals (mouse and rat). The LMF can provide the accessory equipment like heating pads, isofluran vaporizor, puls oximeter, and dental drill for surgery and imaging of narcotized animals.

Zeiss LSM7MP

Zeiss LSM7MP

Our Zeiss LSM7MP is an upright two-photon setup with two infrared lasers, a choice of six non-descanned detectors (NDDs), and three ultra sensitive gallium arsenide phosphide (GaAsP) detectors. The six NDDs and three GaAsPs can be arranged in multiple ways to best suit experiment requirements (reflection and trans NDD configuration). One of the Infrared lasers can be used for imaging while the other one can be used for photo-manipulation. Imaging and photo- manipulation is completely independent from each other due to two pairs of scanning mirrors. The setup is ideally suited for in vivo imaging of narcotized animals (mouse and rat). The LMF can provide the accessory equipment like heating pads, isofluran vaporizor, puls oximeter, and dental drill for surgery and imaging of narcotized animals.

TRIM Scopell with 2 Chameleons

TRIM ScopeII with 2 Chameleons

This system is an upright two-photon setup with two infrared lasers. In the reflection mode it is equipped with three non-descanned detectors (NDDs) and a high sensitivity EM-CCD camera. Two NDDs are ultra sensitive gallium arsenide phosphide (GaAsP) detectors. Both lasers can be used for imaging and photo-manipulation. Furthermore, the system is equipped with LaVision’s 64 beam technology for high speed two-photon microscopy. This technology uses 64 laser beams instead of one to scan the sample and an EM-CCD camera to detect the fluorescence. The system is ideally suited for two-photon imaging in combination with electrophysiology for example in brain slice preparations or narcotized animals (mice). For electrophysiology a Faraday cage, micromanipulators, and an infrared Dodt contrast are installed. The LMF can provide the accessory equipment like heating pads, isofluran vaporizor, puls oximeter, and dental drill for surgery and imaging of narcotized animals.