Spinning disk microscopes are confocal systems with improved Z-resolution like other confocal microscopes. In contrast to confocal laser scanning microscopes, which have only one pinhole spinning disk microscopes have a disk containing multiple pinholes. The pinholes are arranged in such a way that by spinning the disk the whole field of view can be imaged. The disk can spin very fast resulting in an extremely fast confocal microscope.
Andor Spinning Disk Setup
Our spinning disk setup from Andor is built on an inverted fully motorized Nikon microscope with a hardware based focus drift-correction and a fast piezo z-stage. The setup contains a spinning disk from Yokogawa, two back-illuminated high sensitive EM-CCD cameras for simultaneous imaging of two channels, and one sCMOS camera for widefield fluorescence microscopy. Furthermore, a scanning unit for simultaneous imaging and photo-manipulation is complementing the configuration. For imaging and photo-manipulation the setup provides six lasers (405 nm, 445 nm, 488 nm, 515 nm, 561 nm, and 640 nm). The setup has a small incubation chamber for temperature and CO2 control. With this configuration the Andor spinning disk setup is the best choice for fast live cell time-laps recordings including FRET and FRAP experiments.