Stereology is a method that utilizes random, systematic sampling to provide unbiased and quantitative data (i.e. on cell numbers stained by a marker). Stereology has helped to detect many fundamental scientific errors arising from the misinterpretation of plane sections and is considered as gold standard for quantification for example cell numbers.
Widefield Stereology Setup 2
This setup is a stereology setup on a brightfield microscope with the Stereo Investigator software from MicroBrightField. The microscope is a fully motorized upright frame BX-53 (Olympus) equipped with a sensitive black and white as well as a color camera. The setup is best suited for brightfield or fluorescence (no colocalization) stereological investigations with all common stereological procedures (i.e. optical disector or cavalieri)
Spinning Disk Stereology Setup
The spinning disk stereology setup is a fully motorized upright microscope from olympus equipped with the olympus disk spinning unit, a color camera and a very light sensitiv EM-CCD camera (color camera: QImaging Retiga 2000R, 1600 x 1200 pixels, 12 bit, linear full well 40,000 e, readout noise 16 e, QE 55%, 10 frames/sec @ 1600 x 1200 pixel; EM-CCD camera: Hamamatsu C9100-02, 1000 x 1000 pixels, 14 bit, quantum efficiency >
60%, full well capacity 70,000 e, readout noise < 1 e, 30.1 frames/sec @ 1000 x 1000 pixels). The Stereo Investigator software from micro bright field allows performing all common stereological procedures (i.e. optical disector or cavalieri) including widefield, epi-fluorescence and co-localization analysis. The setup can also be used for standard spinning disk or confocal imaging experiments.